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primary human skeletal muscle myoblasts skms  (Lonza)


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    Lonza primary human skeletal muscle myoblasts skms
    Mitochondrial abundance and activity in human <t>primary</t> <t>myoblasts</t> were differentially affected by FFAs. <t>SkMs</t> were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).
    Primary Human Skeletal Muscle Myoblasts Skms, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+skeletal+muscle+myoblasts+skms/pmc08698660-41-0-9?v=Lonza
    Average 90 stars, based on 1 article reviews
    primary human skeletal muscle myoblasts skms - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts"

    Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

    Journal: Biology

    doi: 10.3390/biology10121318

    Mitochondrial abundance and activity in human primary myoblasts were differentially affected by FFAs. SkMs were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).
    Figure Legend Snippet: Mitochondrial abundance and activity in human primary myoblasts were differentially affected by FFAs. SkMs were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).

    Techniques Used: Activity Assay, Control, Incubation, Fluorescence, Software, Comparison

    FFAs do not affect the desmin expression pattern in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) before DAPI and desmin staining. ( b ) Fluorescence images from 3 sets of experiments were analyzed, as described in the legend to . The number of DAPI-positive FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The data are presented as the mean ± SD of cell number (dotted bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).
    Figure Legend Snippet: FFAs do not affect the desmin expression pattern in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) before DAPI and desmin staining. ( b ) Fluorescence images from 3 sets of experiments were analyzed, as described in the legend to . The number of DAPI-positive FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The data are presented as the mean ± SD of cell number (dotted bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Techniques Used: Expressing, Control, Staining, Fluorescence, Comparison

    FFAs are differentially utilized in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) for 48 h and stained using BODIPY. ( b ) Fluorescence images from 3 sets of experiments were analyzed as described in the legend to . The fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of relative lipid accumulation (hatched gray bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*).
    Figure Legend Snippet: FFAs are differentially utilized in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) for 48 h and stained using BODIPY. ( b ) Fluorescence images from 3 sets of experiments were analyzed as described in the legend to . The fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of relative lipid accumulation (hatched gray bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*).

    Techniques Used: Control, Staining, Fluorescence, Comparison

    FFAs selectively activate RTK phosphorylation in human primary myoblasts. SkMs were treated with FAs conjugated with BSA or BSA alone for two hours. Whole-cell lysates were prepared and each incubated with a single RTK array. Following incubation steps, the slides were washed, imaged and analyzed using a laser scanner. Fluorescence images were processed and analyzed simultaneously using analysis software. To obtain the relative phosphorylation (black bars) of individual RTKs indicated at the top of each diagram, integrated signal intensities from FFA-treated cells were compared to that from control cells, which was set to 1 ( a – g , dashed gray line). Data are presented as the mean ± SD of the relative phosphorylation. Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).
    Figure Legend Snippet: FFAs selectively activate RTK phosphorylation in human primary myoblasts. SkMs were treated with FAs conjugated with BSA or BSA alone for two hours. Whole-cell lysates were prepared and each incubated with a single RTK array. Following incubation steps, the slides were washed, imaged and analyzed using a laser scanner. Fluorescence images were processed and analyzed simultaneously using analysis software. To obtain the relative phosphorylation (black bars) of individual RTKs indicated at the top of each diagram, integrated signal intensities from FFA-treated cells were compared to that from control cells, which was set to 1 ( a – g , dashed gray line). Data are presented as the mean ± SD of the relative phosphorylation. Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Techniques Used: Phospho-proteomics, Incubation, Fluorescence, Software, Control, Comparison



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    primary human skeletal muscle myoblasts skms  (Lonza)
    90
    Lonza primary human skeletal muscle myoblasts skms
    Mitochondrial abundance and activity in human <t>primary</t> <t>myoblasts</t> were differentially affected by FFAs. <t>SkMs</t> were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).
    Primary Human Skeletal Muscle Myoblasts Skms, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+skeletal+muscle+myoblasts+skms/pmc08698660-41-0-9?v=Lonza
    Average 90 stars, based on 1 article reviews
    primary human skeletal muscle myoblasts skms - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Mitochondrial abundance and activity in human primary myoblasts were differentially affected by FFAs. SkMs were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).

    Journal: Biology

    Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

    doi: 10.3390/biology10121318

    Figure Lengend Snippet: Mitochondrial abundance and activity in human primary myoblasts were differentially affected by FFAs. SkMs were treated with FAs conjugated with BSA or BSA alone (control). After 48 h, SkMs were incubated with MitoTracker Red ( a ) or Orange ( b ). Fluorescence images at identical settings and exposure times from 3 sets of experiments were simultaneously imported into the analysis software, processed to 8-bit images and analyzed. The integrated fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of the relative fluorescence intensity of MitoTracker Red (white bars) or Orange (black bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*), p ≤ 0.01 (**).

    Article Snippet: Primary human skeletal muscle myoblasts (SkMs) were obtained from Lonza, Basel, Switzerland (CC-2561).

    Techniques: Activity Assay, Control, Incubation, Fluorescence, Software, Comparison

    FFAs do not affect the desmin expression pattern in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) before DAPI and desmin staining. ( b ) Fluorescence images from 3 sets of experiments were analyzed, as described in the legend to . The number of DAPI-positive FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The data are presented as the mean ± SD of cell number (dotted bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Journal: Biology

    Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

    doi: 10.3390/biology10121318

    Figure Lengend Snippet: FFAs do not affect the desmin expression pattern in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) before DAPI and desmin staining. ( b ) Fluorescence images from 3 sets of experiments were analyzed, as described in the legend to . The number of DAPI-positive FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The data are presented as the mean ± SD of cell number (dotted bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Article Snippet: Primary human skeletal muscle myoblasts (SkMs) were obtained from Lonza, Basel, Switzerland (CC-2561).

    Techniques: Expressing, Control, Staining, Fluorescence, Comparison

    FFAs are differentially utilized in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) for 48 h and stained using BODIPY. ( b ) Fluorescence images from 3 sets of experiments were analyzed as described in the legend to . The fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of relative lipid accumulation (hatched gray bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*).

    Journal: Biology

    Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

    doi: 10.3390/biology10121318

    Figure Lengend Snippet: FFAs are differentially utilized in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) for 48 h and stained using BODIPY. ( b ) Fluorescence images from 3 sets of experiments were analyzed as described in the legend to . The fluorescence intensity of FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The results are presented as the mean ± SD of relative lipid accumulation (hatched gray bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*).

    Article Snippet: Primary human skeletal muscle myoblasts (SkMs) were obtained from Lonza, Basel, Switzerland (CC-2561).

    Techniques: Control, Staining, Fluorescence, Comparison

    FFAs selectively activate RTK phosphorylation in human primary myoblasts. SkMs were treated with FAs conjugated with BSA or BSA alone for two hours. Whole-cell lysates were prepared and each incubated with a single RTK array. Following incubation steps, the slides were washed, imaged and analyzed using a laser scanner. Fluorescence images were processed and analyzed simultaneously using analysis software. To obtain the relative phosphorylation (black bars) of individual RTKs indicated at the top of each diagram, integrated signal intensities from FFA-treated cells were compared to that from control cells, which was set to 1 ( a – g , dashed gray line). Data are presented as the mean ± SD of the relative phosphorylation. Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Journal: Biology

    Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

    doi: 10.3390/biology10121318

    Figure Lengend Snippet: FFAs selectively activate RTK phosphorylation in human primary myoblasts. SkMs were treated with FAs conjugated with BSA or BSA alone for two hours. Whole-cell lysates were prepared and each incubated with a single RTK array. Following incubation steps, the slides were washed, imaged and analyzed using a laser scanner. Fluorescence images were processed and analyzed simultaneously using analysis software. To obtain the relative phosphorylation (black bars) of individual RTKs indicated at the top of each diagram, integrated signal intensities from FFA-treated cells were compared to that from control cells, which was set to 1 ( a – g , dashed gray line). Data are presented as the mean ± SD of the relative phosphorylation. Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

    Article Snippet: Primary human skeletal muscle myoblasts (SkMs) were obtained from Lonza, Basel, Switzerland (CC-2561).

    Techniques: Phospho-proteomics, Incubation, Fluorescence, Software, Control, Comparison